EC Number   |
Protein Variants |
Reference |
|---|
   3.4.21.B6 | H85A/D134A/S238A |
protease-dead mutant |
709175 |
| 3.4.21.B6 | more |
construction of Prss8 knockout mutant mice. A T to G substitution, leading to substitution of the active site serine within the catalytic histidine-aspartate-serine triad with alanine, is introduced into exon 6 by site-directed mutagenesis, the clone is injected into strain C57BL/6J blastocysts |
-, 732131 |
| 3.4.21.B6 | more |
prostasin gene silencing, drops epithelial Na+ channel currents to 26% of baseline |
671251 |
| 3.4.21.B6 | more |
prostasin mutant does not manifest the nitric oxide synthase induction attenuation phenotype |
671262 |
| 3.4.21.B6 | R44A |
the mutant cannot be activated by cleavage through hepsin or matriptase |
709925 |
| 3.4.21.B6 | R44Q |
generation of mice expressing zymogen-locked prostasin due to lack of activation site cleavage |
754140 |
| 3.4.21.B6 | S238A |
inactive |
677453 |
| 3.4.21.B6 | S313N/S314L |
lacks the preferred GPI attachment sites and shows decreased activity |
677453 |
| 3.4.21.B6 | S313N/S314L/R322A |
mutant alters both the preferred GPI attachment sites and the potential COOH-terminal tryptic cleavage site, activity is not different from wild type prostasin |
677453 |
