EC Number |
Protein Variants |
Reference |
---|
3.4.21.B48 | D164 |
the mutant shows about 1.3% activity compared to the wild type enzyme |
718028 |
3.4.21.B48 | D164A |
inactive |
718028 |
3.4.21.B48 | D164A |
site-directed mutagenesis, no autoactivation of the proform, inactive mutant |
-, 665094 |
3.4.21.B48 | D164N |
site-directed mutagenesis, replacement of the catalytic residue of kumamolisin-As with that of subtilisin, disrupted interaction of Ser278 with residue 164, the mutant shows 1.3% of the wild-type turnover |
-, 664913 |
3.4.21.B48 | D316A |
site-directed mutagenesis, autoactivation similar to the wild-type enzyme, 3% of wild-type enzyme activity |
-, 665094 |
3.4.21.B48 | D82A |
site-directed mutagenesis, no autoactivation of the proform, inactive mutant |
-, 665094 |
3.4.21.B48 | E32A |
the ratio of turnover number to Km-value for the substrate Lys-Pro-Ile-Ala-Phe-(4-nitro)Phe-Arg-Leu is reduced to 5.7% of the native enzyme |
-, 653914 |
3.4.21.B48 | E78A |
site-directed mutagenesis, no autoactivation of the proform, inactive mutant |
-, 665094 |
3.4.21.B48 | E78A/D164N |
site-directed mutagenesis, completely inactive mutant which stays unprocessed |
-, 664913 |
3.4.21.B48 | E78H |
0.01% activity compared to wild-type enzyme, the canonical catalytic triad is disrupted |
-, 652534 |