EC Number   |
Protein Variants |
Reference |
|---|
   3.4.21.2 | A73T |
diminished activity |
682072 |
| 3.4.21.2 | A73T |
natural missense variant, 62% of wild-type activity |
731886 |
| 3.4.21.2 | D35Y |
natural missense variant, 62% of wild-type activity |
731886 |
| 3.4.21.2 | G18R |
natural missense variant, 84% of wild-type activity |
731886 |
| 3.4.21.2 | G214A |
activity is similar to wild-type enzyme |
754111 |
| 3.4.21.2 | G214M |
activity is similar to wild-type enzyme |
754111 |
| 3.4.21.2 | G214R |
the mutant has increased activity on a small chromogenic peptide substrate but is markedly defective in cleaving bovine beta-casein or the natural chymotrypsin C substrates human cationic trypsinogen and procarboxypeptidase A1. Mutation p.G214R is analogous to the evolutionary mutation in human mesotrypsin, which renders this trypsin isoform resistant to proteinaceous inhibitors and conferrs its ability to cleave these inhibitors. Similarly to the mesotrypsin phenotype, chymotrypsin C variant p.G214R is inhibited poorly by eglin C, ecotin, or a chymotrypsin C-specific variant of SGPI-2, and it readily cleaves the reactive-site peptide bonds in eglin C and ecotin |
754111 |
| 3.4.21.2 | G217R |
natural missense variant, no residual activity |
731886 |
| 3.4.21.2 | G217S |
natural missense variant, 0.5% of wild-type activity |
731886 |
| 3.4.21.2 | G217S |
specific activity is about 7% of the wild type enzyme |
682072 |
