EC Number |
Protein Variants |
Reference |
---|
3.4.21.111 | C194S |
6% of wild type activity at 40°C, less thermostable than the wild type enzyme, similar secondary structure as the wild type enzyme |
-, 680568 |
3.4.21.111 | C5A/C8A |
intracellular distribution similar to wild-type enzyme |
-, 649556 |
3.4.21.111 | C5S/C8S |
intracellular distribution similar to wild-type enzyme |
-, 649556 |
3.4.21.111 | C99S |
69% of wild type activity at 40°C, almost as stable as the wild type enzyme, similar secondary structure as the wild type enzyme |
-, 680568 |
3.4.21.111 | C99S/C194S |
3% of wild type activity at 40°C, less thermostable than the wild type enzyme, similar secondary structure as the wild type enzyme |
-, 680568 |
3.4.21.111 | D138N |
site-sirected mutagenesis |
-, 731114 |
3.4.21.111 | D17N |
site-sirected mutagenesis, the mutant is less thermostable than the wild-type enzyme, although this may be partially due to increased autolysis |
-, 731114 |
3.4.21.111 | D183N |
site-sirected mutagenesis, the disruption of a salt bridge common to proteinase K subfamily enzymes in the D183N mutant results in a significant reduction in thermal stability, and a massive change in the content of the secondary structure compared to the wild-type enzyme |
-, 731114 |
3.4.21.111 | D212N |
site-sirected mutagenesis, thermal stability of D212N is similar to that of the wild-type enzyme at 70°C, but it is inactivated rapidly at 80°C, the mutant is more prone to unfolding at 80°C than the wild-type enzyme |
731114 |
3.4.21.111 | D58N |
site-sirected mutagenesis |
-, 731114 |