EC Number   |
Protein Variants |
Reference |
|---|
    3.4.19.9 | C108A |
complete loss of hydrolytic activity |
732307 |
| 3.4.19.9 | C110A |
inactive mutant enzyme |
647316 |
| 3.4.19.9 | C124A |
Km-value for methotrexate diglutamate is not significantly different from the Km-value of the wild-type enzyme. Specific activity is significantly lower than that of the wild-type enzyme, but the mutant protein has a higher amount of contaminating protein |
647318 |
| 3.4.19.9 | C19A |
Km-value for methotrexate diglutamate is not significantly different from the Km-value of the wild-type enzyme. Specific activity is significantly lower than that of the wild-type enzyme, but the mutant protein has a higher amount of contaminating protein |
647318 |
| 3.4.19.9 | C290A |
Km-value for methotrexate diglutamate is not significantly different from the Km-value of the wild-type enzyme |
647318 |
| 3.4.19.9 | E165A |
mutant shows no poly-gamma-glutamate hydrolysis activity |
732887 |
| 3.4.19.9 | E222A |
maximal velocity with methotrexate diglutamate is reduced 6fold relative to the wild-type enzyme |
647316 |
| 3.4.19.9 | E45A |
mutant shows no poly-gamma-glutamate hydrolysis activity |
732887 |
| 3.4.19.9 | F20A |
about 40% decrease in hyrolytic activity |
732307 |
| 3.4.19.9 | F20A/C108A |
complete loss of hydrolytic activity |
732307 |
