EC Number   |
Protein Variants |
Reference |
|---|
   3.3.2.8 | D101A |
catalytically inactive, D101 is the acid catalyst that protonates the epoxide oxygen |
655334 |
| 3.3.2.8 | D101N |
catalytically inactive, D101 is the acid catalyst that protonates the epoxide oxygen |
655334 |
| 3.3.2.8 | D80A |
site-directed mutagenesis, mutation of the catalytic residue, inactive mutant |
753544 |
| 3.3.2.8 | D82A |
site-directed mutagenesis, mutation of the catalytic residue, inactive mutant |
753544 |
| 3.3.2.8 | E45D/L74F/T76K/M78F/N92K/L114V/I116V |
site-directed mutagenesis, the mutant lacking the N- and C-terminal mutations displays 86% enantiomeric excess in favor of (S,S)-cyclohexene-1,2-diol |
-, 752348 |
| 3.3.2.8 | I5C/S15P/A19K/T76K/E84C/T85V/G89C/S91C/N92K/Y96F/E124D |
site-directed mutagenesis, the multisite mutant shows enhanced and inverted enantioselectivity, and an increase in apparent melting temperature relative to wild-type LEH from 50 to 85°C and a more than 250fold longer half-life |
-, 752348 |
| 3.3.2.8 | I80Y/L114V/I116V |
site-directed mutagenesis, mutant SZ348, no or poor activity with cyclohexene-1,2-epoxide |
753976 |
| 3.3.2.8 | L114C/I116V |
substrate cyclopentene-oxide, 72% conversion, (S,S)-product with 68% enantiomeric excess |
715269 |
| 3.3.2.8 | L114I/I116V |
substrate cyclopentene-oxide, 74% conversion, (S,S)-product with 50% enantiomeric excess |
715269 |
| 3.3.2.8 | L114V/I116V |
substrate cyclopentene-oxide, 72% conversion, (S,S)-product with 60% enantiomeric excess |
715269 |
