EC Number |
Protein Variants |
Reference |
---|
3.2.2.8 | H239A |
dramatic increase in Km for uridine, unchanged kcat |
657373 |
3.2.2.8 | H82N |
small increase in Km, increase in kcat |
657373 |
3.2.2.8 | more |
mutants expressing the Arabidopsis thaliana enzyme or the homologue from Oryza sativa exhibit resistance toward toxic fluorouridine, fluorouracil, and fluoroorotic acid. Mutants with increased and decreased nucleosidase activity are delayed in germination |
700741 |
3.2.2.8 | Q227A |
the mutation causes an increase of kcat for uridine and inosine |
696258 |
3.2.2.8 | Q227F |
the mutation causes an increase of kcat for uridine and inosine |
696258 |
3.2.2.8 | Q227Y |
the mutation has a strong, enhancing effect on the hydrolysis of inosine, and the catalytic efficiency for the purinic substrate is increased by a factor of 7.6 |
696258 |
3.2.2.8 | T223A |
the mutation does not improve significantly the catalytic efficiency of YeiK toward inosine |
696258 |
3.2.2.8 | T223F |
the mutation does not improve significantly the catalytic efficiency of YeiK toward inosine |
696258 |
3.2.2.8 | T223F/Q227Y |
the mutant shows a 2fold increase in catalytic efficiency toward inosine |
696258 |
3.2.2.8 | T223Y |
the mutation does not affect the specificity of the enzyme toward inosine or uridine |
696258 |