EC Number   |
Protein Variants |
Reference |
|---|
   3.2.2.22 | C171A |
does not infer with enzymatic depurination activity, leaving C259 as docking station for the fluorescence dye, which changes its emission properties upon change from aqueous to hydrophobic (membrane) environment, C259 is reduced in the lumen of the endoplasmic reticulum |
698877 |
| 3.2.2.22 | C259A |
decrease in processing of the C-terminal extension |
683982 |
| 3.2.2.22 | D176A |
1.5fold increase in enzyme concentration required for 50% inhibition of protein synthesis |
683935 |
| 3.2.2.22 | D231E |
B chain mutation in one carbohydrate binding site, reduced insecticidal activity, recombinant tobacco plant mutants SNA-I 103M1 and SNA-I 107M1 |
700671 |
| 3.2.2.22 | D75A |
site-directed mutagenesis of the RTA residue, the mutant RTA shows very low expression levels so that purification to homogeneity is not achieved |
664819 |
| 3.2.2.22 | D75N |
site-directed mutagenesis of the RTA residue, the mutant RTA shows very low expression levels so that purification to homogeneity is not achieved |
664819 |
| 3.2.2.22 | D75S |
site-directed mutagenesis of the RTA residue, the mutant RTA shows very low expression levels so that purification to homogeneity is not achieved |
664819 |
| 3.2.2.22 | E167K |
the mutation reduces the toxicity of Shiga toxins 1 and 2 |
716963 |
| 3.2.2.22 | E167K/R176K |
the mutations reduce the toxicity of Shiga toxins 1 and 2 |
716963 |
| 3.2.2.22 | E168D |
replacing DNA fragments of the total synthetic gene of MAP, weaker inhibitory effect |
646898 |
