EC Number |
Protein Variants |
Reference |
---|
3.2.1.25 | D199A |
low activity |
680752 |
3.2.1.25 | D206N |
catalytically active mutant enzyme, similar temperature optimum like wild-type enzyme. The high-catalytic turn-over rate by D206N for beta-glucosidase activity makes it a useful enzyme in cellulose degradation at high temperatures |
750443 |
3.2.1.25 | D206N |
in contrast to the wild-type enzyme which shows a catalytic efficiency for 4-nitrophenyl beta-D-mannopyranoside that is 5fold higher than that for 4-nitrophenyl beta-D-galactopyranoside and 4fold higher than that for 4-nitrophenyl beta-D-glucopyranoside, the mutant enzyme shows highest catalytic efficiency towards 4-nitrophenyl beta-D-glucopyranoside, 11fold higher than that for 4-nitrophenyl beta-D-galactopyranoside and 60fold higher than that for 4-nitrophenyl beta-D-mannopyranoside |
-, 654641 |
3.2.1.25 | D206Q |
mutant enzyme shows less than 10% hydrolytic activity than the wild-type toward 4-nitrophenyl glycosides |
750443 |
3.2.1.25 | E206G |
complete loss of activity |
695664 |
3.2.1.25 | E207S |
no hydrolytic activity |
750443 |
3.2.1.25 | E314G |
complete loss of activity |
695664 |
3.2.1.25 | E330A |
mutant enzyme displays no activity against mannan or 4-nitrophenyl-beta-mannopyranoside |
665524 |
3.2.1.25 | E399S |
no hydrolytic activity |
750443 |
3.2.1.25 | E462A |
low activity |
680752 |