EC Number |
Protein Variants |
Reference |
---|
3.2.1.180 | D115N |
no hydrolysis of beta-D-4-deoxy-DELTA4,5-GlcAp-(1->3)-beta-D-GalNAc6S |
-, 709026 |
3.2.1.180 | D174N |
site-directed mutagenesis |
732164 |
3.2.1.180 | D175N |
no hydrolysis of beta-D-4-deoxy-DELTA4,5-GlcAp-(1->3)-beta-D-GalNAc6S |
-, 709026 |
3.2.1.180 | D182N |
site-directed mutagenesis |
732164 |
3.2.1.180 | F164A |
site-directed mutagenesis |
732164 |
3.2.1.180 | G362Y |
site-directed mutagenesis |
732164 |
3.2.1.180 | K370A |
site-directed mutagenesis |
732164 |
3.2.1.180 | K370I |
kcat/KM for beta-D-4-deoxy-DELTA4,5-GlcAp-(1->3)-beta-D-GalNAc6S is 28% of the wild-type value, kcat/Km for beta-D-4-deoxy-DELTA4,5-GlcAp-(1->3)-beta-D-GalNAc is 5fold higher than wild-type value |
709164 |
3.2.1.180 | R236A |
effectively degrades beta-D-4-deoxy-DELTA4,5-GlcAp-(1->3)-beta-D-GalNAc6S. Almost inactive on beta-D-4-deoxy-DELTA4,5-GlcAp-(1->3)-beta-D-GalNAc4S, although the substrate is degraded by the wild-type enzyme |
-, 709026 |
3.2.1.180 | R236H |
effectively degrades beta-D-4-deoxy-DELTA4,5-GlcAp-(1->3)-beta-D-GalNAc6S. Almost inactive on beta-D-4-deoxy-DELTA4,5-GlcAp-(1->3)-beta-D-GalNAc4S, although the substrate is degraded by the wild-type enzyme |
-, 709026 |