EC Number   |
Protein Variants |
Reference |
|---|
    3.2.1.177 | C307I |
isoprimeverose-hydrolyzing activity of the mutant enzyme is reduced 400fold compared to wild-type enzyme. alpha-Glucosidase activity is not detected |
715011 |
| 3.2.1.177 | C307I/F308D |
alpha-xylosidase activity of wild-type enzyme is converted into alpha-glucosidase activity. Mutant shows 140fold higher hydrolytic activity against p-nitrophenyl alpha-glucopyranoside than wild-type YicI. The mutant is able to hydrolyze nigerose [alpha-D-Glcp-(1->3)-D-Glcp] and kojibiose [alpha-D-Glcp-(1->2)-D-Glcp], which are not hydrolyzed by the wild-type enzyme. Isoprimeverose-hydrolyzing activity of the L1Chi mutant is decreased by about 3700fold, and that of C307I/F308D is diminished to below the limits of detection. alpha-Glucosidase activity with 4-nitrophenyl alpha-D-glucoside is increased 132fold compared to wild-type activity |
715011 |
| 3.2.1.177 | D659A |
site-directed mutagenesis, active site mutant, catalytically inactive variant |
731657 |
| 3.2.1.177 | F277Y |
isoprimeverose-hydrolyzing activity of the mutant enzyme is reduced 20fold compared to wild-type enzyme. alpha-Glucosidase activity is not detected (alpha-glucosidase activity of the wild-type enzyme is 96244fold lower than specific activity with isoprimeverose) |
715011 |
| 3.2.1.177 | K414W |
isoprimeverose-hydrolyzing activity of the mutant enzyme is reduced 12000fold compared to wild-type enzyme. alpha-Glucosidase activity is not detected |
715011 |
| 3.2.1.177 | W345I |
isoprimeverose-hydrolyzing activity of the mutant enzyme is reduced 16000fold compared to wild-type enzyme. alpha-Glucosidase activity is not detected |
715011 |
