EC Number |
Protein Variants |
Reference |
---|
3.2.1.165 | D139A |
the mutation significantly decreases the hydrolytic activity compared to the wild type enzyme. The mutant enzyme exclusively exhibits glycosynthase activity using alpha-D-glucosaminyl fluoride and D-glucosamine as substrates |
-, 749852 |
3.2.1.165 | D139E |
compared with the wild type, the hydrolytic activity of the mutant is significantly suppressed (less than 0.1%), and the fluoride release activity also decreases to less than 3% |
-, 749852 |
3.2.1.165 | D139S |
the mutation significantly decreases the hydrolytic activity compared to the wild type enzyme |
-, 749852 |
3.2.1.165 | D143A |
the mutation significantly decreases the hydrolytic activity compared to the wild type enzyme |
-, 749852 |
3.2.1.165 | D178N |
inactive |
751103 |
3.2.1.165 | D180N |
inactive |
751103 |
3.2.1.165 | D464A |
specific acitivity is 0.44% compared to the activity of the wild-type enzyme |
748421 |
3.2.1.165 | D464E |
no activity |
748421 |
3.2.1.165 | D464E/E539D |
no activity |
748421 |
3.2.1.165 | D464N |
specific acitivity is 1.9% compared to the activity of the wild-type enzyme |
748421 |