EC Number   |
Protein Variants |
Reference |
|---|
   3.2.1.157 | D247A |
the enzymatic activity is completely abolished |
714214 |
| 3.2.1.157 | D359N |
the mutant shows reduced activity compared to the wild type enzyme. Asp359 is not critical for catalysis |
-, 716089 |
| 3.2.1.157 | E245Q |
the enzymatic activity is completely abolished |
714214 |
| 3.2.1.157 | E310Q |
the Km value is reduced by a factor of about 4, the reduction in kcat by 50fold results in a substantial decrease in the overall enzyme efficiency (about 10fold) |
714214 |
| 3.2.1.157 | H281A |
the mutant shows 7fold decreased Km and 180fold decreased kcat value compared to the wild type enzyme with 4% relative efficiency |
714214 |
| 3.2.1.157 | Q222E |
the enzymatic activity is abolished |
714214 |
| 3.2.1.157 | Q222K |
the enzymatic activity is abolished |
714214 |
