EC Number   |
Protein Variants |
Reference |
|---|
    3.2.1.141 | A259S |
mutation increases selectivity ratio glucose/trehalose formation |
692727 |
| 3.2.1.141 | A259S |
site-directed mutagenesis, the mutant shows increased selectivity ratios, i.e. ratios of the catalytic efficiencies for glucose formation to those for trehalose formation in the hydrolysis of maltooligosaccharides and maltooligosyltrehaloses, respectively |
-, 692726 |
| 3.2.1.141 | D156A |
kcat/Km for maltooligosyltrehalose is 3.8fold lower than wild-type value |
-, 726343 |
| 3.2.1.141 | D252E |
mutant shows 0.03% of enzymatic activity compared to the wild-type |
-, 726451 |
| 3.2.1.141 | D252S |
mutant shows 0.6% of enzymatic activity compared to the wild-type |
-, 726451 |
| 3.2.1.141 | D255A |
0.15% of wild-type activity |
692727 |
| 3.2.1.141 | D255A |
site-directed mutagenesis, the mutant shows highly reduced catalytic activity compared to the wild-type MTHase |
-, 692726 |
| 3.2.1.141 | D380A |
0.01% of wild-type activity |
692727 |
| 3.2.1.141 | D380A |
site-directed mutagenesis, the mutant shows highly reduced catalytic activity compared to the wild-type MTHase |
-, 692726 |
| 3.2.1.141 | E283Q |
mutant shows 0.04% of enzymatic activity compared to the wild-type |
-, 726451 |
