EC Number |
Protein Variants |
Reference |
---|
3.2.1.131 | AguADELTAC |
lacks the last 81 amino acids, heat labile, 0.1% of the specific activity of the wild-type enzyme at 35°C |
646735 |
3.2.1.131 | AguADELTAN |
lacks the first 126 amino acids, 1% of the specific activity of the wild-type enzyme at 55°C |
646735 |
3.2.1.131 | AguADELTANC |
lacks the first 126 and last 81 amino acids, is insoluble |
646735 |
3.2.1.131 | D192A |
the mutant shows wild type activity |
-, 732167 |
3.2.1.131 | D206A |
the mutant shows strongly reduced activity compared to the wild type enzyme |
-, 732167 |
3.2.1.131 | D215A |
15fold decrease in catalytic efficiency |
738687 |
3.2.1.131 | D274A |
no change in activity |
646735 |
3.2.1.131 | D332A |
inactive |
-, 732167 |
3.2.1.131 | D335A |
complete loss of activtiy |
738687 |
3.2.1.131 | D335A |
weak binding of substrate |
738687 |