EC Number   |
Protein Variants |
Reference |
|---|
   3.2.1.111 | 249DELTA265 |
the mutant shows decreased activity with 4-nitrophenyl alpha-L-fucoside (14fold) 3-fucosyllactose (5fold) and Lewis X trisaccharide (9fold) compared to the wild type enzyme |
751107 |
| 3.2.1.111 | C317A |
the mutant shows decreased activity with 4-nitrophenyl alpha-L-fucoside (9fold) 3-fucosyllactose (4fold) and Lewis X trisaccharide (6fold) compared to the wild type enzyme |
751107 |
| 3.2.1.111 | C317S |
the mutant shows decreased activity with 4-nitrophenyl alpha-L-fucoside (2fold) 3-fucosyllactose (3fold) and Lewis X trisaccharide (2fold) compared to the wild type enzyme |
-, 751107 |
| 3.2.1.111 | C467S |
the mutant shows decreased activity with 4-nitrophenyl alpha-L-fucoside (0.9fold) 3-fucosyllactose (0.9fold) and Lewis X trisaccharide (9fold) compared to the wild type enzyme |
751107 |
| 3.2.1.111 | C73S |
the mutant shows wild type activity with 4-nitrophenyl alpha-L-fucoside and Lewis X trisaccharide and shows decreased activity with 3-fucosyllactose (2fold) compared to the wild type enzyme |
-, 751107 |
| 3.2.1.111 | D242A |
the mutant is inactive with 3-fucosyllactose and shows decreased activity with 4-nitrophenyl alpha-L-fucoside (10000fold) and Lewis X trisaccharide (1928fold) compared to the wild type enzyme |
-, 751107 |
| 3.2.1.111 | E302A |
the mutant is inactive with 3-fucosyllactose and shows decreased activity with 4-nitrophenyl alpha-L-fucoside (736fold) and Lewis X trisaccharide (900fold) compared to the wild type enzyme |
-, 751107 |
| 3.2.1.111 | E315A |
the mutant is inactive with 4-nitrophenyl alpha-L-fucoside and shows decreased activity with 3-fucosyllactose (473fold) and Lewis X trisaccharide (585fold) compared to the wild type enzyme |
-, 751107 |
| 3.2.1.111 | F34H/L321P |
the mutant exhibits increased transfucosylation activity compared to the wild type enzyme |
-, 750709 |
| 3.2.1.111 | F34I/L321P |
the mutant exhibits increased transfucosylation activity compared to the wild type enzyme |
-, 750709 |
