EC Number   |
Protein Variants |
Reference |
|---|
   3.1.8.2 | Asp229/Asn120 |
site-directed mutagenesis, the mutant shows impaired catalytic activity or decreased substrate binding affinity |
752291, 755573 |
| 3.1.8.2 | Asp229/Asn175 |
site-directed mutagenesis, the mutant shows impaired catalytic activity or decreased substrate binding affinity |
752291, 755573 |
| 3.1.8.2 | D121E |
the mutant displays 87% activity compared to the wild type enzyme |
703171 |
| 3.1.8.2 | D121F |
no activity |
664118 |
| 3.1.8.2 | D229N |
enzymatically inactive |
703171 |
| 3.1.8.2 | D229N/N120D |
catalytically inactive, no change in the calcium coordinating environment |
680347 |
| 3.1.8.2 | D229N/N120D |
no activity |
664118 |
| 3.1.8.2 | D229N/N175D |
catalytically inactive, no change in the calcium coordinating environment |
680347 |
| 3.1.8.2 | D232S |
3% higher activity than the wild-type |
664118 |
| 3.1.8.2 | E21Q/N120D |
catalytically inactive |
680347 |
