EC Number |
Protein Variants |
Reference |
---|
3.1.30.2 | G133V |
yield after proteinase K treatment 0% |
698091 |
3.1.30.2 | G139V |
yield after proteinase K treatment 0% |
698091 |
3.1.30.2 | H168A |
yield after proteinase K treatment 0% |
698091 |
3.1.30.2 | H171A |
yield after proteinase K treatment 0% |
698091 |
3.1.30.2 | H237A |
yield after proteinase K treatment 20% |
698091 |
3.1.30.2 | H89G |
hydroxylamine reduces the growth of the recombinant strain Escherichia coli TGE900 pHisNucSma (H89G) compared to the control without hydroxylamine. Such a decrease in growth may be due to the restoration of the activity of the mutant enzyme, leading to the cleavage of nucleic acids in the cell |
751382 |
3.1.30.2 | K235A |
yield after proteinase K treatment 19% |
698091 |
3.1.30.2 | K235R |
yield after proteinase K treatment 21% |
698091 |
3.1.30.2 | R121/122A |
yield after proteinase K treatment 4% |
698091 |
3.1.30.2 | R121A |
for specific activity measurement |
698091 |