EC Number |
Protein Variants |
Reference |
---|
3.1.3.B4 | C392S |
catalytically inactive mutant |
716168 |
3.1.3.B4 | C392S |
inactive mutant does not rescue the defect of Sac1 deletion |
732981 |
3.1.3.B4 | C392S |
mutant is stable and displays a profile similar to that of the wild-type protein as determined by gel filtration chromatography. Mutant shows no activity |
731293 |
3.1.3.B4 | C392S |
mutation leads to inactivation |
732218 |
3.1.3.B4 | C458S |
catalytically inactive |
751173 |
3.1.3.B4 | D394N |
mutant is stable and displays a profile similar to that of the wild-type protein as determined by gel filtration chromatography. Mutant shows no activity |
731293 |
3.1.3.B4 | D397N |
mutant is stable and displays a profile similar to that of the wild-type protein as determined by gel filtration chromatography. Mutant shows no activity |
731293 |
3.1.3.B4 | DELTA2-460 |
a truncated Sac1 mutant (Sac1(2-460), truncated at the end of the structured region) exhibits essentially no PtdIns4P phosphatase activity. In contrast to full-length version, presence of PtdIns4P in liposomes does not elicit substantial membrane binding by the truncated mutant Sac1(2-460), suggesting that residues 461-511 are important for substrate recognition |
732218 |
3.1.3.B4 | G449S |
no activity |
694636 |
3.1.3.B4 | more |
usage of the recombinant Sac1p Sac1 phosphatase activity to deplete phosphatidylinositol-4-phosphate from semi-intact cell membranes, method development and optimization, overview |
716168 |