EC Number |
Protein Variants |
Reference |
---|
3.1.3.75 | C87A |
involved in a non-essential intramolecular disulfide bond |
706604 |
3.1.3.75 | C94A |
involved in a non-essential intramolecular disulfide bond |
706604 |
3.1.3.75 | D123N |
site-directed mutagenesis, the mutant enzyme fragment shows reduced activity compared to the wild-type enzyme fragment |
664211 |
3.1.3.75 | D203S |
site-directed mutagenesis, inactive mutant enzyme fragment |
664211 |
3.1.3.75 | D262E |
low PChP activity |
679380 |
3.1.3.75 | D262E |
mutant shows activity less than 2% with respect to native recombinant enzyme |
696420 |
3.1.3.75 | D265E |
enzyme activity reduced to 30-35% of wild-type activity |
679380 |
3.1.3.75 | D267E |
low PChP activity |
679380 |
3.1.3.75 | D267E |
mutant shows activity less than 2% with respect to native recombinant enzyme and is not activated by Cu2+ |
696420 |
3.1.3.75 | D31E |
protein expression, but no enzyme activity |
679380 |