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Protein Variants:
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EC Number
Protein Variants
Commentary
Reference
3.1.3.15
D12A
shows only traces of the wild type activity
680722
3.1.3.15
D17N
inactive
-
,
729227
3.1.3.15
D213A
site-directed mutagenesis
-
,
751106
3.1.3.15
D228N
the mutant shows reduced catalytic efficiency and kcat is reduced by approximately 6000fold compared to the wild type enzyme
729227
3.1.3.15
D44A
site-directed mutagenesis
-
,
751106
3.1.3.15
D83A
site-directed mutagenesis
-
,
751106
3.1.3.15
E115Q
the mutant shows reduced catalytic efficiency compared to the wild type enzyme
-
,
729227
3.1.3.15
E18A
shows about 5% of the wild-type activity
680722
3.1.3.15
E67A
site-directed mutagenesis
-
,
751106
3.1.3.15
G195R
conserved Gly, required for histinol-phosphate phosphatase activity
706327
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