EC Number   |
Protein Variants |
Reference |
|---|
   3.1.21.6 | D19A |
relative activity of bottom-strand cleavage is less than half that of top-strand cleavage, whereas activity of top-strand cleavage is more than 80% that of the wild-type |
682175 |
| 3.1.21.6 | E147A |
does not exhibit a significant reduction in nicking activity, compared with that of the wild-type |
682175 |
| 3.1.21.6 | E18A |
relative activity of bottom-strand cleavage is less than half that of top-strand cleavage, whereas activity of top-strand cleavage is more than 80% that of the wild-type |
682175 |
| 3.1.21.6 | H130A |
moderately decreased nicking activity |
682175 |
| 3.1.21.6 | H140A |
shows less than 20% of the wild-type activity for the cleavage of both strands, amounts of cleavage products are less than 1% of the total substrates |
682175 |
| 3.1.21.6 | H209A |
exhibits no detectable cleavage activity on the top or bottom strand of the oligonucleotide substrate |
682175 |
| 3.1.21.6 | H54A |
relative activity of bottom-strand cleavage is less than half that of top-strand cleavage, whereas activity of top-strand cleavage is more than 80% that of the wild-type |
682175 |
| 3.1.21.6 | H78A |
shows less than 20% of the wild-type activity for the cleavage of both strands, amounts of cleavage products are less than 1% of the total substrates |
682175 |
| 3.1.21.6 | K56A |
moderately decreased nicking activity |
682175 |
| 3.1.21.6 | N180A |
altered cleavage patterns, shows less than 20% of the wild-type activity for the cleavage of both strands, amounts of cleavage products are less than 1% of the total substrates |
682175 |
