EC Number |
Protein Variants |
Reference |
---|
3.1.2.29 | E50A |
disturbed protein folding. 37% of wild-type catalytic efficiency, substrate inhibition above 0.019 mM |
712448 |
3.1.2.29 | E50A |
putative member of the catalytic triad |
712448 |
3.1.2.29 | E50Q |
about 5000fold decrease in catalytic efficiency |
711265 |
3.1.2.29 | F33A |
about 1000fold decrease in catalytic efficiency |
711265 |
3.1.2.29 | F36A |
about 100fold decrease in catalytic efficiency |
711265 |
3.1.2.29 | F36A |
the mutation results in a change in KM by affecting substrate binding |
752061 |
3.1.2.29 | H76A |
about 100000fold decrease in catalytic efficiency |
711265 |
3.1.2.29 | H76A |
the kinetic isotope effec observed with [2H2]fluoroacetyl-CoA is abolished in the H76A mutant, showing that H76 is directly involved in Calpha deprotonation |
730813 |
3.1.2.29 | H76D |
insoluble protein |
712448 |
3.1.2.29 | H76D |
putative member of the catalytic triad, completely insoluble |
712448 |