EC Number   |
Protein Variants |
Reference |
|---|
   3.1.2.23 | D17E |
site-directed mutagensis, increased activity |
-, 650103 |
| 3.1.2.23 | D17N |
site-directed mutagensis, highly reduced activity |
-, 650103 |
| 3.1.2.23 | D17N |
site-directed mutagensis, role of Asp17 in ligand binding |
-, 652279 |
| 3.1.2.23 | D17S |
site-directed mutagensis, highly reduced activity |
-, 650103 |
| 3.1.2.23 | D31A |
steady-state kinetic analysis |
729220 |
| 3.1.2.23 | D31N |
steady-state kinetic analysis |
729220 |
| 3.1.2.23 | E73A |
steady-state kinetic analysis, crystallization data |
-, 729220 |
| 3.1.2.23 | E73D |
mutation switches the function of the carboxylate residue from nucleophilic catalysis to base catalysis and thus, the reaction from a two-step process involving a covalent enzyme intermediate to a single-step hydrolysis reaction |
729220 |
| 3.1.2.23 | E73D |
steady-state kinetic analysis |
729220 |
| 3.1.2.23 | E73D/T77A |
mutant regains most of the catalytic efficiency lost in the E73D single mutant |
729220 |
