Any feedback?
Please rate this page
(search_result.php)
(0/150)

BRENDA support

Refine search

Search Protein Variants

show results
Don't show organism specific information (fast!)
Search organism in taxonomic tree (slow, choose "exact" as search mode, e.g. "mammalia" for rat,human,monkey,...)
(Not possible to combine with the first option)
Refine your search

Search term:

Results 1 - 10 of 14 > >>
EC Number Protein Variants Commentary Reference
Display the word mapDisplay the reaction diagram Show all sequences 3.1.13.3D130A pJS94, encoding the catalytically inactive point mutant OrnA D130A does not suppress the phenotype of the Streptomyces coelicolor J3411 (DELTAornA::apr) null mutant 697978
Display the word mapDisplay the reaction diagram Show all sequences 3.1.13.3D163A site-directed mutagenesis, mutation of the catalytic residue, inactive mutant 752249
Display the word mapDisplay the reaction diagram Show all sequences 3.1.13.3H158A site-directed mutagenesis, the mutant completely loses its hydrolytic cleavage ability -, 752249
Display the word mapDisplay the reaction diagram Show all sequences 3.1.13.3H66A site-directed mutagenesis, mutation is located on the alpha2 helix, inactive mutant 752249
Display the word mapDisplay the reaction diagram Show all sequences 3.1.13.3more construction of an enzyme knockout mutant by gene replacement, Pseudomonas aeruginosa DELTAorn mutant has high intracellular cyclic diguanylate (c-di-GMP) levels, causing this strain to overexpress extracellular polymers and overproduce biofilm. Mutant DELTAorn cells possess highly elevated 5'-phosphoguanylyl-(3',5')-guanosine (pGpG) levels -, 752033
Display the word mapDisplay the reaction diagram Show all sequences 3.1.13.3more generation of enzyme mutant DELTAorn, mRNA levels of pyocin biosynthesis genes in the DELTAorn mutant compared to those in wild-type strain PA14. Mutation of orn drastically increases bacterial susceptibility to quinolones but not to tetracycline, aminoglycoside, or beta-lactam antibiotics. Upregulation of pyocin genes contributes to increased susceptibility to ciprofloxacin in the orn mutant. PrtR stability is reduced by the mutation of orn 749595
Display the word mapDisplay the reaction diagram Show all sequences 3.1.13.3more generation of enzyme mutant DELTAorn. The lysates from DELTAorn show 25fold decrease in 5'-phosphoguanylyl-(3',5')-guanosine (pGpG) hydrolysis. Complementation with wild-type, but not active site mutants, restores hydrolysis. Accumulation of pGpG in the DELTAorn strain inhibits PDE-As, increasing cyclic-diguanylate (c-di-GMP) concentration. Increased transcription from the cyclic-diguanylate-regulated pel promoter is observed. Additionally, the cyclic-diguanylate-governed auto-aggregation and biofilm phenotypes are elevated in the DELTAorn strain in a pel-dependent manner. Detection of elevated levels of pGpG and cyclic-diguanylate in the DELTAorn strain 752038
Display the word mapDisplay the reaction diagram Show all sequences 3.1.13.3more oligoribonuclease null mutant J3411 (DELTAornA::apr), reveals a conditional defect in morphological differentiation that is reminiscent of bldA. PpJS93, containing ornA under the control of a constitutive promoter, completely suppresses the phenotype of Streptomyces coelicolor J3411 (DELTAornA::apr), but pJS94 (encoding catalytically inactive OrnA D130A) does not 697978
Display the word mapDisplay the reaction diagram Show all sequences 3.1.13.3more ORN mutant, shows growth defect, YtqI from Bacillus subtilis is able to complement the mutant 682178
Display the word mapDisplay the reaction diagram Show all sequences 3.1.13.3more ytqI mutant, shows impairment of growth in the absence of cysteine 682178
Results 1 - 10 of 14 > >>