EC Number |
Protein Variants |
Reference |
---|
3.1.13.3 | D130A |
pJS94, encoding the catalytically inactive point mutant OrnA D130A does not suppress the phenotype of the Streptomyces coelicolor J3411 (DELTAornA::apr) null mutant |
697978 |
3.1.13.3 | D163A |
site-directed mutagenesis, mutation of the catalytic residue, inactive mutant |
752249 |
3.1.13.3 | H158A |
site-directed mutagenesis, the mutant completely loses its hydrolytic cleavage ability |
-, 752249 |
3.1.13.3 | H66A |
site-directed mutagenesis, mutation is located on the alpha2 helix, inactive mutant |
752249 |
3.1.13.3 | more |
construction of an enzyme knockout mutant by gene replacement, Pseudomonas aeruginosa DELTAorn mutant has high intracellular cyclic diguanylate (c-di-GMP) levels, causing this strain to overexpress extracellular polymers and overproduce biofilm. Mutant DELTAorn cells possess highly elevated 5'-phosphoguanylyl-(3',5')-guanosine (pGpG) levels |
-, 752033 |
3.1.13.3 | more |
generation of enzyme mutant DELTAorn, mRNA levels of pyocin biosynthesis genes in the DELTAorn mutant compared to those in wild-type strain PA14. Mutation of orn drastically increases bacterial susceptibility to quinolones but not to tetracycline, aminoglycoside, or beta-lactam antibiotics. Upregulation of pyocin genes contributes to increased susceptibility to ciprofloxacin in the orn mutant. PrtR stability is reduced by the mutation of orn |
749595 |
3.1.13.3 | more |
generation of enzyme mutant DELTAorn. The lysates from DELTAorn show 25fold decrease in 5'-phosphoguanylyl-(3',5')-guanosine (pGpG) hydrolysis. Complementation with wild-type, but not active site mutants, restores hydrolysis. Accumulation of pGpG in the DELTAorn strain inhibits PDE-As, increasing cyclic-diguanylate (c-di-GMP) concentration. Increased transcription from the cyclic-diguanylate-regulated pel promoter is observed. Additionally, the cyclic-diguanylate-governed auto-aggregation and biofilm phenotypes are elevated in the DELTAorn strain in a pel-dependent manner. Detection of elevated levels of pGpG and cyclic-diguanylate in the DELTAorn strain |
752038 |
3.1.13.3 | more |
oligoribonuclease null mutant J3411 (DELTAornA::apr), reveals a conditional defect in morphological differentiation that is reminiscent of bldA. PpJS93, containing ornA under the control of a constitutive promoter, completely suppresses the phenotype of Streptomyces coelicolor J3411 (DELTAornA::apr), but pJS94 (encoding catalytically inactive OrnA D130A) does not |
697978 |
3.1.13.3 | more |
ORN mutant, shows growth defect, YtqI from Bacillus subtilis is able to complement the mutant |
682178 |
3.1.13.3 | more |
ytqI mutant, shows impairment of growth in the absence of cysteine |
682178 |