EC Number   |
Protein Variants |
Reference |
|---|
  3.1.1.56 | C60H/W197I |
increased kcat and similar KM for p-nitrophenyl substrates compared to mutant W197I, increased phoshorylation by diethyl p-nitrophenyl phosphate |
691005 |
| 3.1.1.56 | C60K/W197I |
similar kcat and KM for p-nitrophenyl substrates compared to mutant W197I, increased phoshorylation by diethyl p-nitrophenyl phosphate, 11°C decreased melting temperature compared to mutant W197I |
691005 |
| 3.1.1.56 | C60Q/W197I |
decreased catalytic activity, but no affect on KM for p-nitrophenyl acetate compared to mutant W197I |
691005 |
| 3.1.1.56 | C60R/W197I |
similar kcat and KM for p-nitrophenyl substrates compared to mutant W197I, increased phoshorylation by diethyl p-nitrophenyl phosphate, 10°C decreased melting temperature compared to mutant W197I |
691005 |
| 3.1.1.56 | C60S/W197I |
double mutant |
691005 |
| 3.1.1.56 | D226A |
4.3% of wild-type activity |
697853 |
| 3.1.1.56 | D226N |
9% of wild-type activity |
697853 |
| 3.1.1.56 | G57H |
decreased catalytic activity, enhanced dephosphorylation rate, close to oxyanion hole |
691005 |
| 3.1.1.56 | G57H/W197I |
catalytically inactive |
691005 |
| 3.1.1.56 | H260A |
3% of wild-type activity |
697853 |
