EC Number |
Protein Variants |
Reference |
---|
3.1.1.45 | A150T |
site-directed mutagenesis, the mutant shows slightly higher Vmax with olmesartan medoxomil compared to the wild-type enzyme |
729629 |
3.1.1.45 | C123A |
inactive |
94355 |
3.1.1.45 | C123S |
- |
94357 |
3.1.1.45 | C123S |
100% activity towards alpha-naphthyl acetate compared with the wild type enzyme |
-, 671042 |
3.1.1.45 | C123S |
burst kinetics with p-nitrophenyl acetate, 10% as active as DLH |
94355 |
3.1.1.45 | C123S |
maximal activity 20% that of the wild type protein |
94356, 94358 |
3.1.1.45 | C123S |
site-directed mutagenesis, mutation of the active site cysteine to a serine, giving a catalytic triad found in serine proteases, completely changes the catalytic activity to a dienelactone isomerase |
-, 650835 |
3.1.1.45 | C123S/R206A |
65% activity towards alpha-naphthyl acetate compared with the wild type enzyme |
671042 |
3.1.1.45 | C132A |
mutant shows a drastic reduction of the olmesartan medoxomil-hydrolyzing activity |
704653 |
3.1.1.45 | C132A |
mutant shows a low activity, 30% of wild-type |
704653 |