EC Number   |
Protein Variants |
Reference |
|---|
   3.1.1.29 | C166A |
site-directed mutagenesis, the mutant effectively complements the enzyme-defective thermosensitive Escherichia coli mutant strain AA7852 for growth at 42°C |
-, 678535 |
| 3.1.1.29 | C67S |
site-directed mutagenesis, the mutant effectively complements the enzyme-defective thermosensitive Escherichia coli mutant strain AA7852 for growth at 42°C |
-, 678535 |
| 3.1.1.29 | C703A |
mutation abolishes editing activity |
721151 |
| 3.1.1.29 | D86A |
kcat/Km for diacetyl-lysyl-tRNALys is 0.22% of wild-type value |
664102 |
| 3.1.1.29 | D86A/K18A |
kcat/Km for diacetyl-lysyl-tRNALys is 0.17% of wild-type value |
664102 |
| 3.1.1.29 | D93A |
turnover-number is 0.1% of the turnover-number for wild-type enzyme, Km-value for diacetyl-Lys-tRNALys is 1.67fold higher than the Km-value for the wild-type enzyme |
648118 |
| 3.1.1.29 | D93N |
4% of wild-type kcat |
650249 |
| 3.1.1.29 | D95N |
site-directed mutagenesis, the catalytic residue mutant is not able to complement the enzyme-defective thermosensitive Escherichia coli mutant strain AA7852 for growth at 42°C |
-, 678535 |
| 3.1.1.29 | D97N |
site-directed mutagenesis, the catalytically important hydrogen bond between D97 and H24 is lost after the mutation and a new H-bond is formed between H24 and N118 |
752162 |
| 3.1.1.29 | F66A |
turnover-number is 26% of the turnover-number for wild-type enzyme, Km-value for diacetyl-Lys-tRNALys is 1.15fold higher than the Km-value for the wild-type enzyme |
648118 |
