EC Number   |
Protein Variants |
Reference |
|---|
    2.8.1.2 | C154S |
active site mutant |
723958 |
| 2.8.1.2 | C154S |
SH group titration, susceptibility to hydrogen peroxide and tetrathionate and renaturation by dithiothreitol similar to wild-type |
662415 |
| 2.8.1.2 | C154S |
site-directed mutagenesis, expression in Escherichia coli BL21 (DE3), overexpressed, structurally resembles an active form of MST |
674808 |
| 2.8.1.2 | C247S |
inactive |
724231 |
| 2.8.1.2 | C247S |
no enzyme activity |
645563 |
| 2.8.1.2 | C247S |
SH group titration, susceptibility to hydrogen peroxide and tetrathionate and renaturation by dithiothreitol similar to wild-type |
662415 |
| 2.8.1.2 | C247S |
site-directed mutagenesis, transient transfection of human embryonic kidney HEK 293-F cells, mutant completely lose the activity to metabolize 3-mercaptopyruvate that is assessed by measuring its products either pyruvate or H2S, the levels of bound sulfane sulfur are not increased |
690476 |
| 2.8.1.2 | C248R |
slightly increased Km |
645563 |
| 2.8.1.2 | C254S |
SH group titration, susceptibility to hydrogen peroxide and tetrathionate and renaturation by dithiothreitol similar to wild-type |
662415 |
| 2.8.1.2 | C254S |
site-directed mutagenesis, expression in Escherichia coli BL21 (DE3), overexpressed, activation with reduced thioredoxin |
674808 |
