EC Number   |
Protein Variants |
Reference |
|---|
   2.7.7.72 | A75C |
as compared to wild-type enzyme the Km increases 2.7-fold from A addition to C addition, while the kcat decreased 14fold. The overall kcat/Km ratio of C addition to A addition is 0.03 |
724262 |
| 2.7.7.72 | D106A |
CTP-adding activity is 90% of wild-type level, ATP-adding activities is 14% of wild-type level |
725334 |
| 2.7.7.72 | D106A |
mutation has no effect on CCA-adding activity |
725334 |
| 2.7.7.72 | D124A |
mutation has no effect on CCA-adding activity |
725334 |
| 2.7.7.72 | D139A |
mutant shows a strong reduction in the addition of the terminal A position |
739631 |
| 2.7.7.72 | D143A |
mutation has no effect on CCA-adding activity |
725334 |
| 2.7.7.72 | D215A |
CTP-adding activity is 16% of wild-type level, ATP-adding activities is 13% of wild-type level |
725334 |
| 2.7.7.72 | D215A |
mutation has no effect on CCA-adding activity |
725334 |
| 2.7.7.72 | D218A |
mutation has no effect on CCA-adding activity |
725334 |
| 2.7.7.72 | D53A |
no activity |
725334 |
