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EC Number	Protein Variants	Commentary	Reference
2.7.7.71	S53N	site-directed mutagenesis, the mutant is constructed to mimic the UTP binding site of PpMurU, but GTP is still the major cofactor for the nucleotidylyltransferase activity for the mutant	761308
2.7.7.71	S53V/E80Q	site-directed mutagenesis, the mutant is constructed to mimic the nucleotide-binding pocket of the UDPGP family, the GTP affinity is severely diminished without increment of pyrimidine affinity	761308

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Release 2023.1 (January 2023)