EC Number   |
Protein Variants |
Reference |
|---|
    2.7.7.69 | G224D |
less than 0.1% of wild-type activity |
693081 |
| 2.7.7.69 | H238N |
less than 0.1% of wild-type activity |
693081 |
| 2.7.7.69 | more |
analysis of the phenotype of two SlGGP1-deficient EMS Micro-Tom mutants. Fruits of the ggp1 plants produce more ethylene and show higher firmness and soluble solids content than the wild-type after the breaker stage. Leaf CO2 uptake decreases about 50% in both ggp1 mutants at saturating light conditions; however, O2 production in an enriched CO2 atmosphere is only 19% higher in wild-type leaves. Leaf conductance that is largely reduced in both mutants may be the main limitation for photosynthesis. Ethylene production is higher in the mutant lines than wild-type fruit at the breaker and red stages |
762186 |
| 2.7.7.69 | more |
Chlamydomonas reinhardtii strain cw15-325 is used as recipient strain for transformation with VTC2 amiRNA. The VTC2 gene encoding GDP-L-galactose phosphorylase is targeted using artificial microRNAs. Upon H2O2 stress, alanine and proline accumulated, whereas the amount of cystine strongly decreases in the VTC2 amiRNA lines. The amounts of aspartic acid, lysine and ornithine are lower in the VTC2 amiRNA lines under control conditions, but these differences are much smaller following H2O2 stress. Changes in the tricarboxylic acid cycle intermediates are also apparent. Following H2O2 treatment, the amount of pyruvic acid and citric acid decreases, but the amounts of malic acid and succinic acid increases in all the lines. Furthermore, the amount of mannose and glycerol-3-phosphate decreases in the VTC2 amiRNA lines. Phenotype analysis, overview |
-, 762022 |
| 2.7.7.69 | more |
construction of hemizygous and homozygous OsGGP overexpressing plants using the constitutive dual CaMV 35S promoter and Agrobacterium-mediated transformation leading to 5-9fold increased ascorbate concentrations in homozygous plants compared to low amounts in heterozygous and wild-type plants. Phenotype analysis, overview. Ascorbate concentrations are positively correlated with ferritin cn Caco-2 cells exposed to in vitro digests of NS and 35S-OsGGP BR and GBR samples |
761175 |
| 2.7.7.69 | more |
construction of isoforms Vtc2/Vtc5 double mutants. Double mutants show growth arrest immediately upon germination and the cotyledons subsequently bleach. Normal growth is restored by supplementation with ascorbate or L-galactose |
694667 |
| 2.7.7.69 | more |
construction of isoforms Vtc2/Vtc5 double mutants. Double mutants show growth arrest immediately upon germination and the cotyledons subsequently bleach. Normal growth is restored by supplementation with ascorbate or L-galactose. vtc2-1 leaves contain more mannose 6-phosphate than wild-type |
694667 |
| 2.7.7.69 | S290F |
less than 0.1% of wild-type activity |
693081 |
