EC Number |
Protein Variants |
Reference |
---|
2.7.7.14 | H146A |
site-directed mutagenesis, the mutant is almost inactive |
721549 |
2.7.7.14 | H226A |
mutation in histidine residue in the HxGH motif of the C-terminal CT domain. Decrease in activity |
737597 |
2.7.7.14 | H229A |
mutation in histidine residue in the HxGH motif of the N-terminal CT domain. Activity similar to wild-type activity |
737597 |
2.7.7.14 | H244A |
inactive C-domain mutant, presence of the mutant reduces splice variant Pcyt2alpha homodimerization and activity |
738322 |
2.7.7.14 | H244Y |
inactive C-domain mutant, presence of the mutant reduces splice variant Pcyt2alpha homodimerization and activity |
738322 |
2.7.7.14 | H35A |
inactive N-domain mutant, presence of the mutant reduces splice variant Pcyt2alpha homodimerization and activity |
738322 |
2.7.7.14 | H35A |
mutation in histidine residue in the HxGH motif of the N-terminal CT domain. Complete loss of activity |
737597 |
2.7.7.14 | H35Y |
inactive N-domain mutant, presence of the mutant reduces splice variant Pcyt2alpha homodimerization and activity |
738322 |
2.7.7.14 | H38A |
mutation in histidine residue in the HxGH motif of the N-terminal CT domain. Strong decrease in activity |
737597 |
2.7.7.14 | H422A |
site-directed mutagenesis, the mutant shows increased kcat and Vmax with ethanolamine phosphate compared to the wild-type enzyme |
721549 |