EC Number   |
Protein Variants |
Reference |
|---|
    2.7.7.13 | D27H/V330I |
mutation identified in patient with muscular dystrophy |
737417, 741569 |
| 2.7.7.13 | K175E |
approx. 9% of wild-type activity, 600fold increase in Km for mannose 1-phosphate |
2823 |
| 2.7.7.13 | K175Q |
approx. 40% of wild-type activity, 3200fold increase in Km for mannose 1-phosphate |
2823 |
| 2.7.7.13 | K175R |
470fold increase in mannose 1-phosphate Km value |
2823 |
| 2.7.7.13 | K20Q |
enzyme is unable to support alginate synthesis although it shows no significant differences in Vmax and Km as compared to wild-type |
2823 |
| 2.7.7.13 | more |
construction of several truncated mutants of the PH0925 protein, that show altered kinetics compared to the wild-type enzyme, overview |
-, 733811 |
| 2.7.7.13 | more |
construction of the conditional inactivation mutant strain YJ-gmpp by replacement of the native Afsrb1 promoter with an inducible Aspergillus nidulans alcA promoter. Repression of GMPP in yeast leads to phenotypes including hyphal lysis, defective cell wall, and failure of polarized growth and branching site selection, and cell separation, inactivation phenotype, overview. The alkali-soluble alpha-glucan and alkali-insoluble beta-glucan contents remain unchanged in strain YJ-gmpp depleted of Afsrb1, while the alkali-soluble protein and alkali-insoluble chitin contents are 1.3 and 2.0fold higher, respectively, than those of the wild-type |
-, 700044 |
| 2.7.7.13 | more |
generation of GMP3 overexpressing and RNAi knockdown lines |
739463 |
| 2.7.7.13 | more |
generation of OsVTC1-1 silenced mutant plants by RNAi method |
739330 |
| 2.7.7.13 | more |
generation of OsVTC1-3 silenced mutant plants by RNAi method |
739330 |
