EC Number   |
Protein Variants |
Reference |
|---|
   2.7.4.22 | A122T |
activity decreased to 26% of the wild-type |
666266 |
| 2.7.4.22 | D113A |
the mutant shows an increased Km value for UMP compared to the wild type enzyme |
-, 723289 |
| 2.7.4.22 | D115A |
little detrimental effect, activated by 5'-guanylyl-imidodiphosphate within a concentration range that is roughly similar to that of wild type enzyme |
703708 |
| 2.7.4.22 | D146N |
84% of wild-type activity in the pellet of the sonicated bacterial extract |
665362 |
| 2.7.4.22 | D159N |
appears at neutral pH almost exclusively as a hexamer |
663998 |
| 2.7.4.22 | D159N |
exhibits higher solubility than the wild-type protein at neutral pH, 10% of wild-type activity in the pellet of the sonicated bacterial extract |
665362 |
| 2.7.4.22 | D159N |
site-directed mutagenesis |
698858 |
| 2.7.4.22 | D159N |
used as reference enzyme |
698858 |
| 2.7.4.22 | D168N |
97% of wild-type activity in the pellet of the sonicated bacterial extract |
665362 |
| 2.7.4.22 | D168N |
as stable as wild-type |
663823 |
