EC Number |
Protein Variants |
Reference |
---|
2.7.4.13 | D16N |
site-directed mutagenesis of the P loop-related residue, the mutant shows reduced activity and altered substrate specificity compared to the wild-type enzyme |
739645 |
2.7.4.13 | D170N |
site-directed mutagenesis of the core residue, almost inactive mutant |
739645 |
2.7.4.13 | E176Q |
site-directed mutagenesis of the core residue, almost inactive mutant |
739645 |
2.7.4.13 | G137A |
site-directed mutagenesis of the dNMP-binding residue, the mutant shows highly reduced activity and altered substrate specificity compared to the wild-type enzyme |
739645 |
2.7.4.13 | K131E |
site-directed mutagenesis, of the dNMP-binding residue, the mutant shows slightly increased activity and altered substrate specificity compared to the wild-type enzyme |
739645 |
2.7.4.13 | more |
all enzyme mutants but T17S show an increased pH optimum compared to the wild-type enzyme |
739645 |
2.7.4.13 | Q134A |
site-directed mutagenesis of the dNMP-binding residue, the mutant shows reduced activity and altered substrate specificity compared to the wild-type enzyme |
739645 |
2.7.4.13 | R130K |
site-directed mutagenesis of the dNMP-binding residue, almost inactive mutant |
739645 |
2.7.4.13 | R172I |
site-directed mutagenesis of the core residue, almost inactive mutant |
739645 |
2.7.4.13 | S13A |
site-directed mutagenesis of the P loop-related residue, the mutant shows reduced activity and altered substrate specificity compared to the wild-type enzyme |
739645 |