EC Number |
Protein Variants |
Reference |
---|
2.7.3.4 | A59G |
site-directed mutagenesis on domain 1, the mutant shows a high decrease in affinity and activity for taurocyamine compared to the wild-type enzyme |
738180 |
2.7.3.4 | A62G |
site-directed mutagenesis on domain 2, inactive mutant |
738180 |
2.7.3.4 | C268S |
site-directed mutagenesis |
738646 |
2.7.3.4 | C268S/C631S |
site-directed mutagenesis |
738646 |
2.7.3.4 | C631S |
site-directed mutagenesis |
738646 |
2.7.3.4 | G58R |
site-directed mutagenesis on domain 1, the mutant shows slightly decreased affinity for taurocyamine and a slightly increased activity for taurocyamine compared to the wild-type enzyme |
738180 |
2.7.3.4 | H61A |
site-directed mutagenesis of a domain 2 residue, the enzyme mutant shows altered kinetics and reduced activity compared to the wild-type enzyme |
739402 |
2.7.3.4 | H61A |
site-directed mutagenesis of TKD1D2 in D2 region, the enzyme mutant shows altered kinetics and reduced activity compared to the wild-type enzyme |
739402 |
2.7.3.4 | H67A |
the mutant shows a decreased Km value compared to the wild type enzyme |
721715 |
2.7.3.4 | H67A/K95Y |
the mutant shows significantly decreased Km value compared to the wild type enzyme |
721715 |