EC Number |
Protein Variants |
Reference |
---|
2.7.2.11 | A226S |
the mutant has 1.2fold increased activity and higher thermostability compared to the wild type enzyme |
-, 760828 |
2.7.2.11 | A62T |
drastic reduction in specific activity. 911fold increase in Ki-value for L-Pro compared to wild-type enzyme |
642208 |
2.7.2.11 | A62V |
drastic reduction in specific activity. 188fold increase in Ki-value for L-Pro compared to wild-type enzyme |
642208 |
2.7.2.11 | D107A |
mutant shows 40fold increased IC50 (L-proline) |
722974 |
2.7.2.11 | D137A |
mutation hampers proline binding and glutamate binding, IC50 (L-proline) 142fold increased compared to wild-type |
722974 |
2.7.2.11 | D147G |
reduction in catalytic activity. 2000fold increase in Ki-value for L-Pro compared to wild-type enzyme |
642208 |
2.7.2.11 | D148A |
is active, 150fold increased proline requirement |
679845 |
2.7.2.11 | D148N |
is active, 40fold increased proline requirement |
679845 |
2.7.2.11 | D150A |
is not active |
679845 |
2.7.2.11 | D150N |
is active |
679845 |