EC Number   |
Protein Variants |
Reference |
|---|
   2.7.1.150 | D2134R |
site-directed mutagenesis, in vivo abrogation of lipid kinase activity of the enzyme |
660790 |
| 2.7.1.150 | G1867/1870V |
site-directed mutagenesis, the Cgfab1DELTA mutant carrying CgFab1G1867/1870V is sensitive to both azole and metal ion stress |
761922 |
| 2.7.1.150 | K2059M |
site-directed mutagenesis, in vitro abrogation of lipid kinase activity of the enzyme |
660790 |
| 2.7.1.150 | more |
enzyme defect leads to enlarged vacuoles that do not acidify correctly, the defect can be complemented by enzyme overexpression, mutants grow poorly at elevated temperature |
661530 |
| 2.7.1.150 | more |
FAB1 null mutants possess no phosphatidylinositol 3,5-bisphosphate and shows vacuolar defects, expression of murine enzyme PIKfyve, but of the enzyme from Schizosaccharomyces pombe, can revert the vacuolar defects in vivo, while the enzyme from Schizosaccharomyces pombe can restore catalytic activity in response to hyperosmotic stress |
660790 |
| 2.7.1.150 | more |
generation of a deletion strain for the CgFAB1 gene, using the homologous recombination-based strategy. The mutant is sensitive to azoles (fluconazole, clotrimazole and ketoconazole), and cell membrane (SDS) and cell wall (caffeine) stressors. The growth of the Cgfab1DELTA mutant is also slightly impaired in the presence of the oxidative stressor, hydrogen peroxide, and at high temperature. MDR genes are activated in the Cgfab1DELTA mutant upon fluconazole exposure |
761922 |
| 2.7.1.150 | more |
identification of a non-functional ste12PIKFYVE mutant that is unable to invoke the normal advancement of mitotic onset and adjust cell size at division in response to nitrogen stress |
-, 762218 |
| 2.7.1.150 | more |
knockdown of FAB1A/B expression |
739357 |
| 2.7.1.150 | more |
ppk-3(n2668) strong loss-of-function mutants embryos contain many vacuolar structures of different sizes, a subset of which are positive for both LAAT-1::GFP and HIS-24::mCh, indicating that they are phagolysosomes. The double mutant of slc-36.1(yq110) with ppk-3(n2668) contains enlarged autolysosomes similar to ppk-3(n2668) single mutants |
761580 |
| 2.7.1.150 | S1448L |
naturally occuring mutation yq24, yq24 mutants exhibit embryonic vacuoles similar to slc-36.1 mutants. Double mutants of yq24 with ced-4(n1162) show neither button-like apoptotic cell corpses nor vacuolar structures. The yq24 embryonic vacuoles are enriched for both LAAT-1::GFP and HIS-24::mCh. Mutant yq24 embryonic vacuoles are phagolysosomes arising from apoptosis |
761580 |
