EC Number |
Protein Variants |
Reference |
---|
2.7.1.108 | G405S |
naturally occuring mutation, identified in the isolated mutant strain MD2/1-9, the mutation leads to reduced dolichol kinase activity and the enhanced secretion phenotype |
-, 761104 |
2.7.1.108 | G405S/I419S |
site-directed mutagenesis, despite some glycosylation defects, double DK mutations (G405S and I419S) in the Kluyveromyces lactis mutant strain demonstrate three times the level of recombinant alpha-amylase secretion compared to wild-type strain. Overexpression of potential suppressors KlMNN10, KlSEL1, KlERG20, KlSRT1, KlRER2, KlCAX4, KlLPP1 and KlDPP1 in the DK-mutant strain restores carboxypeptidase Y glycosylation to different extents and, with the exception of KISRT1, reduces alpha-amylase secretion to levels observed in wild-type cells |
-, 761104 |
2.7.1.108 | G471D |
site-directed mutagenesis,inactive mutant |
761619 |
2.7.1.108 | I419S |
naturally occuring mutation, identified in the isolated mutant strain MD2/1-9, the mutation leads to reduced dolichol kinase activity and the enhanced secretion phenotype |
-, 761104 |
2.7.1.108 | more |
construction of two independent lines of T-DNA-tagged AtDOK1 mutants, designated dok1-1 (CS822420; SAIL_529_E06) and dok1-2 (CS856101; WiscDsLox443D6), with T-DNA insertions in exons 8 and 13, respectively. Mutant dok1-1/+ and wild-type seed morphologies show no significant differences, and dok1-1/+ and the wild type did not differ in seed germination rates |
739314 |
2.7.1.108 | more |
creation of leaky knockdown mutants of gene DOK1 by microRNA-mediated gene suppression technique. Knockout of gene DOK1 causes lethality, while the DOK1 knockdown mutants show an early flowering phenotype without any remarkable growth defect in vegetative tissues |
761619 |
2.7.1.108 | more |
phenotypic characterization of CBS2359-KlSEC59 mutants, overview |
-, 761104 |
2.7.1.108 | Q483K |
naturally occuring homozygous c.1447C>A DOLK mutation involved in enzyme deficiency and a phenotype with anatomic malformations and multi-systemic dysfunction |
739079 |
2.7.1.108 | W332G |
mutated Sec59-1p bears the single amino acid substitution Trp(332) to Gly, region predicted to be responsible for the substrate (dolichol) binding |
-, 677274 |