EC Number |
Protein Variants |
Reference |
---|
2.6.1.B16 | F84A |
site-directed mutagenesis, substrate specificity analysis |
759888 |
2.6.1.B16 | F84G |
site-directed mutagenesis, substrate specificity analysis |
759888 |
2.6.1.B16 | F84L |
site-directed mutagenesis, substrate specificity analysis |
759888 |
2.6.1.B16 | F84W |
site-directed mutagenesis, substrate specificity analysis |
759888 |
2.6.1.B16 | G51S |
site-directed mutagenesis, the ATA-v2 mutant shows superior operational, temperature and solvent stability as well as improved activity for the pharmaceutical relevant amine product 4-phenyl-2-butanamine appears to be specific for thermal tolerance, compared to wild-type, mutant ATA-v1 shows increased temperature optimum, and features excellent operational stability in biphasic (aqueous/nonpolar solvent) reaction systems at 45°C, when the aqueous phase contained an approximate amine donor-to-acceptor ratio |
758672 |
2.6.1.B16 | I258A |
site-directed mutagenesis, substrate specificity analysis |
759888 |
2.6.1.B16 | I258G |
site-directed mutagenesis, substrate specificity analysis |
759888 |
2.6.1.B16 | I258V |
site-directed mutagenesis, substrate specificity analysis |
759888 |
2.6.1.B16 | more |
comparison of pH-profile and amino acceptor substrate specificity of mutants R162A and R410A and wild-type Ban-TA |
758672 |
2.6.1.B16 | more |
development of an efficient bioproduction of amines by exploiting the immobilised (S)-selective amine transaminase from Halomonas elongata (HEWT), which shows a very broad substrate scope, tolerating a range of temperatures, pH values, salts, and co-solvents, in a continuous flow-reactor. The immobilised HEWT (imm-HEWT) is applied to continuous-flow reactions maintaining the biocatalyst in a packed-bed reactor to decrease reaction times and increase the productivity. The addition of an in-line purification step allows for the recovery of the pure products without any additional work-up procedure. Method optimization and evaluation, overview |
759320 |