2.6.1.27 | more |
an auxin-defective mutant, tillering and small grain 1 (tsg1), displays an increased number of tillers but reduced panicle and grain size due to mutation of TSG1, which encodes a tryptophan aminotransferase. TSG1 is allelic to the previously identified FISH BONE (FIB) gene, loss-of-function which results in pleiotropic abnormal phenotypes. The TSG1 gene, an F2 population derived from a cross between the tsg1 mutant line and japonica rice variety Zhonghua-11 (ZH11), is obtained using the map-based cloning approach. The TSG1 locus is originally mapped to the long arm of chromosome 1 between marker loci G01856 and G011812 using 636 homozygous F2 plants then subsequently fine-mapped to a 26-kb region between marker loci G011422 and G011436, which contains two open reading frames (ORFs), LOC_Os01g07490 and LOC_Os01g07500. Knockout of TSG1 using clustered regularly interspaced short palindromic repeats (CRISPR)/CRISPR-associated protein 9 (Cas9) genome editing in ZH11-produced plants reveal a phenotype similar to that of the natural tsg1 mutant. Phenotypes, overview |
759589 |