EC Number |
Protein Variants |
Reference |
---|
2.5.1.9 | A43L |
decrease in affinity for substrate 6,7-dimethyl-8-ribityllumazine. A43L replacement causes substantial perturbation of the overall binding site topology |
687468 |
2.5.1.9 | C48A |
site-directed mutagenesis, nearly inactive mutant |
658346 |
2.5.1.9 | C48M |
site-directed mutagenesis, nearly inactive mutant |
658346 |
2.5.1.9 | C48S |
mutation in the activity cavity, causes significant 19F NMR chemical shift modulation of trifluoromethyl derivatives of 6,7-dimethyl-8-ribityllumazine in complex with the protein. Replacement of C48 changes the electron density topology in the N-terminal substrate binding site in the vicinity of C-6 and C-7 atoms of bound ligand |
687468 |
2.5.1.9 | C48S |
site-directed mutagenesis, highly decreased activity compared to the wild-type enzyme |
658346 |
2.5.1.9 | D143G |
site-directed mutagenesis, soluble protein, too unstable to be purified |
637580 |
2.5.1.9 | D143N |
site-directed mutagenesis, soluble protein, too unstable to be purified |
637580 |
2.5.1.9 | D185L |
site-directed mutagenesis, low remaining activity |
637580 |
2.5.1.9 | DELTA1-180 |
variant that lacks the C-terminal extension the coiled-coil and C-terminal peptide (AaRS1-180) |
759481 |
2.5.1.9 | DELTA1-196 |
variant that lacks the C-terminal extension (1-196). Substantial decrease in association efficiency with lumazine synthase is observed for the truncated variant |
759481 |