EC Number   |
Protein Variants |
Reference |
|---|
    2.5.1.81 | D109A |
mutation leads to the production of longer prenyl diphosphates from C30 to C35, but at small rates |
721495 |
| 2.5.1.81 | I112A |
chain length of the products of mutant I112A reaches up to C70 or longer, which is three times as long as that of the wild type. A large fraction of the longer products is C45-50, which is nearly twice as long as wild-type |
721495 |
| 2.5.1.81 | II116A |
mutation leads to the production of longer prenyl diphosphates from C30 to C35, but at small rates |
721495 |
| 2.5.1.81 | M137A |
slight increase in chain length of products |
721495 |
| 2.5.1.81 | M72A |
slight increase in chain length of products |
721495 |
| 2.5.1.81 | more |
directed evolution is used to create mutant FGPP synthases that confirm the importance of amino acids upstream of the FARM of prenyl diphosphate synthases and demonstrate the significance of mutations upstream of an additional conserved region (141GQ142). Product chain-length distribution can be also controlled by a structural change provoked by a cooperative interaction of amino acids |
660416 |
| 2.5.1.81 | S175M |
the mutated AtIDS7 enzyme produces mainly geranylgeranyl diphosphate and only small quantities of geranylfarnesyl diphosphate |
-, 739320 |
