EC Number   |
Protein Variants |
Reference |
|---|
    2.5.1.7 | C115A |
site-directed mutagenesis, overexpression in Escherichia coli, no activity |
-, 637598 |
| 2.5.1.7 | C115D |
mutant protein is functional and resistant to fosfomycin and inhibitors 2-[4-(2-hydroxyethyl)piperazin-1-yl]-6,7-dimethoxy-3,4-dihydronaphthalen-1(2H)-one and2-(4-methylpiperazin-1-yl)-3,4-dihydronaphthalen-1(2H)-one |
685595 |
| 2.5.1.7 | C115D |
site-directed mutagenesis, overexpression in Escherichia coli, gains fosfomycin resistance, forms only the phospholactyl-enzyme intermediate adduct, but no UDP-GlcNAc-phosphoenolpyruvate, higher kcat than the wild-type at pH 7.0, enhanced pH-dependency of the reaction |
-, 637598 |
| 2.5.1.7 | C115D |
the mutant enzyme lacks the ability to react with phosphoenolpyruvate covalently |
722746 |
| 2.5.1.7 | C115E |
site-directed mutagenesis, overexpression in Escherichia coli, gains fosfomycin resistance, enhanced pH-dependency of the reaction, low activity |
-, 637598 |
| 2.5.1.7 | C115N |
site-directed mutagenesis, overexpression in Escherichia coli, deamination of Asn115 to Asp115 |
-, 637598 |
| 2.5.1.7 | C115S |
- |
-, 637598, 637602, 637603, 637605 |
| 2.5.1.7 | C115S |
mutant enzyme is capable of catalyzing the wild type enzyme reaction but is unable to release the products (single turnover), activity is not affected by fosfomycin |
674498 |
| 2.5.1.7 | C115S |
site-directed mutagenesis, overexpression in Escherichia coli, no activity |
-, 637598 |
| 2.5.1.7 | C117D |
the mutant is not inhibited by fosfomycin |
738855 |
