EC Number   |
Protein Variants |
Reference |
|---|
    2.5.1.43 | E81Q |
mutant binds the substrate but not the product |
706512 |
| 2.5.1.43 | E81Q |
site-directed mutagenesis, inactive mutant enzyme |
722028 |
| 2.5.1.43 | E81Q/Y107F |
inactive mutant |
706512 |
| 2.5.1.43 | L115A/L116A |
isozyme OsNAS2 mutated in the LL motif resulting in mutant m7-sGFP, which does not localize to the vesicles. Mutant m7sGFP does not show NAS enzyme activity |
739307 |
| 2.5.1.43 | more |
breeding nas T-DNA insertion mutants leads to mutants without NAS activity (named nas4x-2, sterile, strongly chlorotic) and with reduced NAS activity especially during the reproductive phase (named nas4x-1, leaves, roots, and flowers with symptoms of iron deficiency) |
706307 |
| 2.5.1.43 | more |
enhanced expression of OsNAS2 gene results in elevated nicotianamide levels, greater Zn accumulations, and improved plant tolerance to a Zn deficiency, the expression of Zn-uptake genes and those for the biosynthesis of phytosiderophores are increased in transgenic plants, phenotypes overview. In the endosperm, the OsNAS2 activation-tagged line contain up to 20fold more nicotianamide and 2.7fold more zinc |
723378 |
| 2.5.1.43 | more |
generation of a mutant Salk 135507 carrying a T-DNA insertion in gene AtNAS4 |
-, 739365 |
| 2.5.1.43 | more |
generation of transgenic Oryza sativa plants by expression of iron-inducible nicotianamine synthase for increasing the content of bioavailable iron in seeds. Enhanced expression leads to higher tolerance of Fe deficiency and better growth under elevated pH, the iron content is increased 3fold by activating the recombinant NAS, phenotypes, overview |
723191 |
| 2.5.1.43 | more |
isozyme OsNAS2 mutated in the YXXphi motif, mutant m6-sGFP, is localized to the vesicles. These vesicles stuck together and are immobile. OsNAS2 mutated in the LL motif, mutant m7-sGFP, does not localize to these vesicles |
739374 |
| 2.5.1.43 | more |
mutant enzyme phenotype under both Fe-sufficient and -deficient conditions, overview |
739307 |
