EC Number   |
Protein Variants |
Reference |
|---|
   2.5.1.3 | H341L |
no activity |
-, 727098 |
| 2.5.1.3 | M134K |
phosphomethylpyrimidine kinase activity similar to wild-type, thiamin-phosphate diphosphorylase activity almost completely abolished |
671903 |
| 2.5.1.3 | M134K/T472D |
83-97% residual phosphomethylpyrimidine kinase activity, 19-32% residual thiamin-phosphate diphosphorylase activity |
671903 |
| 2.5.1.3 | more |
in enzyme deletion mutant, no growth difference is observed when compared to the wild-type strain |
688400 |
| 2.5.1.3 | Q373L |
phosphomethylpyrimidine kinase activity similar to wild-type, 64-75% residual thiamin-phosphate diphosphorylase activity |
671903 |
| 2.5.1.3 | Q98L |
phosphomethylpyrimidine kinase activity similar to wild-type, thiamin-phosphate diphosphorylase activity almost completely abolished |
671903 |
| 2.5.1.3 | Q98L/Q373L |
phosphomethylpyrimidine kinase activity similar to wild-type, 7-13% residual thiamin-phosphate diphosphorylase activity |
671903 |
| 2.5.1.3 | R320M |
activity is 1% compared to wild-type enzyme |
-, 727098 |
| 2.5.1.3 | S444A |
almost complete loss of phosphomethylpyrimidine kinase activity, 80-91% residual thiamin-phosphate diphosphorylase activity |
671903 |
| 2.5.1.3 | T472D |
phosphomethylpyrimidine kinase activity similar to wild-type, 46-54% residual thiamin-phosphate diphosphorylase activity |
671903 |
