EC Number   |
Protein Variants |
Reference |
|---|
    2.5.1.19 | A100G |
glyphosate-sensitive, compared to wild-type similar inhibition constants for (3R,4S,5R)-5-[(2R)-1,1-difluoro-3-hydroxy-3-oxo-2-phosphonooxy-propan-2-yl]oxy-4-hydroxy-3-phosphonooxy-cyclohexene-1-carboxylic acid, (3R,4S,5R)-4-hydroxy-5-[(2R)-1-hydroxy-1-oxo-2-phosphono-propan-2-yl]oxy-3-phosphonooxy-cyclohexene-1-carboxylic acid, and (3R,4S,5R)-4-hydroxy-5-[(2S)-1-hydroxy-1-oxo-2-phosphono-propan-2-yl]oxy-3-phosphonooxy-cyclohexene-1-carboxylic acid |
-, 690885 |
| 2.5.1.19 | A183T |
38fold decrease in phosphoenolpyruvate-binding affinity, more solvent-exposed tryptophan residues and lower stability against guanidine hydrochloride compared to wild-type and mutant G96A, midpoint guanidine hydrochloride concentration of unfolding: 0.7 M, higher structural flexibility and decrease of secondary structure (28% alpha helix, 35% beta sheet) compared to wild-type (40% alpha helix, 31% beta sheet) and lowest resistance against proteolysis, residue A183 located on the exterior in the N-terminal domain |
691087 |
| 2.5.1.19 | A187T |
the mutation alters glyphosate resistance and slightly reduces enzyme activity |
739266 |
| 2.5.1.19 | A242V |
the mutation leads to sensitivity to 8.768% glyphosate |
758550 |
| 2.5.1.19 | A329T |
the mutation leads to sensitivity to 8.768% glyphosate |
-, 758550 |
| 2.5.1.19 | agriculture |
genetically engineered rice endogenous enzyme gene overexpressing 5-enolpyruvoylshikimate-3-phosphate synthase can increase glyphosate herbicide-resistance of cultivated rice. This type of enzyme transgene can enhance the fecundity of rice crop-weed hybrid offspring in the absence of glyphosate, stimulating great concerns over undesired environmental impacts of transgene flow to populations of wild relatives |
760158 |
| 2.5.1.19 | agriculture |
glyphosate resistance is correlated with the degree of enzyme over-expression in plants |
760025 |
| 2.5.1.19 | agriculture |
the enzyme is efficiently utilized to develop transgenic glyphosate-tolerant crops |
739671 |
| 2.5.1.19 | D242A |
site-directed mutagenesis, the mutation is responsible for the high increase in activity |
658298 |
| 2.5.1.19 | D313A |
0.42% of wild-type activity |
638211 |
