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Protein Variants:
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EC Number
Protein Variants
Commentary
Reference
2.5.1.153
D34A
compared to wild-type, mutant produces about 1% (13S)-isotuberculosinol and 60% tuberculosinol
-
,
729897
2.5.1.153
D81N
24% activity compared to the wild type enzyme
-
,
714542
2.5.1.153
D81N/D82N
inactive
-
,
714542
2.5.1.153
D81N/D85N
inactive
-
,
714542
2.5.1.153
D82N
21% activity compared to the wild type enzyme
-
,
714542
2.5.1.153
D82N/D85N
inactive
714542
2.5.1.153
D85N
37% activity compared to the wild type enzyme
-
,
714542
2.5.1.153
R38A
compared to wild-type, mutant produces about 5% (13S)-isotuberculosinol and 140% tuberculosinol
-
,
729897
2.5.1.153
Y51F
production of both (13S)-isotuberculosinol and tuberculosinol is reduced
-
,
729897
2.5.1.153
Y51F/Y90F
no enzymic activity
-
,
729897
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