EC Number   |
Protein Variants |
Reference |
|---|
    2.4.2.1 | A117T |
mutation leading to purine-nucleoside phosphorylase deficiency, occuring in two sisters |
703563 |
| 2.4.2.1 | A196E/D238N |
the mutations in the trimeric enzyme form change substrate specificity from guanosine to adenosine |
-, 736075 |
| 2.4.2.1 | C254S/C256S |
after denaturation using 6 M guadinium HCl, mutant achieves a recovery of catalytic activity of 46% |
703662 |
| 2.4.2.1 | C254S/C256S |
reduced thermodynamic and kinetic stability of the mutant with respect to the wild-type PfPNP |
673591 |
| 2.4.2.1 | C254S/C256S |
Tm-value is 8°C lower than Tm-value of wild-type enzyme |
673591 |
| 2.4.2.1 | D204A |
0.1% of wild-type activity towards inosine, adenosine and guanosine |
721756 |
| 2.4.2.1 | D204A/R217A |
about 0.2% of wild-type activity towards inosine, adenosine and guanosine |
721756 |
| 2.4.2.1 | D204N |
less than about 4% of wild-type activity towards inosine, adenosine and guanosine |
721756 |
| 2.4.2.1 | D204N |
site-directed mutagenesis, the enzymatic ribosylation of 1,N6-etheno-isoguanine using Escherichia coli PNP wild-type and D204N mutant enzymes gives different products, which are identified on the basis of NMR analysis and comparison with the product of the isoguanosine reaction with chloroacetic aldehyde, which gives an essentially single compound, identified unequivocally as N9-riboside |
759805 |
| 2.4.2.1 | D204N |
site-directed mutagenesis, the mutant exhibits altered substrate specificity compared to wild-type |
759799 |
